PacBio：How HiFi sequencing works

Sequencing free of systematic error achieves 99.9% read accuracy and >90% of bases Q30+. Explore the benefits of highly accurate long-read sequencing. With exceptional accuracy, low sequencing-context bias, and accurate mapping of reads, HiFi sequencing provides the information needed to confidently call and detect all variants.

Data from a 15 kb size-selected human library using the SMRTbell express template prep kit 2.0 on a Sequel IIe system (2.0 chemistry, Sequel IIe system software v10, 30-hour movie). Read lengths, reads/data per SMRT Cell, and other sequencing performance results vary based on sample quality/type and insert size.