Illumina adapter sequences:What sequences do I use for adapter trimming

What sequences do I use for adapter trimming

What sequences do I use for adapter trimming

ThefulladaptersequencesforvariousIlluminalibrarypreparationkitscanbefoundintheIlluminaAdapterSequencesDocument.IlluminaStrandedmRNAPrep, ...。其他文章還包含有:「Illuminaadapterportfolio」、「IlluminaAdapterSequences」、「IlluminaAdapterSequences(1000000002694)」、「IlluminaAdapterSequencesDocument(1000000002694...」、「WhataretheIlluminasequencingprimersequences?」

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WhenperformingsequencingonanIlluminainstrument,sequencescorrespondingtothelibraryadapterscanbepresentintheFASTQfilesatthe3’endofthereads[1]ifthereadlengthislongerthantheinsertsize.Toremovethesesequencesandpreventissueswithdownstreamalignment,adaptertrimmingisanoptioninIlluminaFASTQgenerationpipelines.SamplesheetsgeneratedwithIlluminaExperimentManager[2]containthenecessarysequencesintheSettingssectionforIlluminakits.IlluminakitsinBaseSpace™SequenceHubPrep,BaseSpaceSequenceHubInstrumentRunSetu...