NCBI Primer-BLAST：Primer designing tool
Primer designing tool
A tool for finding specific primers
https://www.ncbi.nlm.nih.gov
To find such primers, it uses BLAST to align the longest sequence among the group to the rest to find common regions which are then used to limit the locations ...
NCBI Minute
https://www.youtube.com
Primer
https://www.ncbi.nlm.nih.gov
Primer-BLAST to better identify the template and thus perform better primer specificity checking. A template is not required if both forward and reverse ...
Primer
https://www.ncbi.nlm.nih.gov
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target. It uses Primer3 to design PCR primers and then uses ...
Primer-BLAST
https://bmcbioinformatics.biom
Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers.
Using NCBI BLAST to locate primers
https://www.idtdna.com
Want to quickly find the location of primers within a gene or the expected size of the resultant PCR product? In this tip we show you how with NCBI's BLAST.
設計primer流程(捷登）
http://llai.cm.ntu.edu.tw
使⽤用時機：. 1. qPCR 時使⽤用（片段較短，<250 bp）. 2. ⽤用於⼀一般PCR 驗證. 3. ⽤用於質體建構時的target gene 製備(需恰好使⽤用整段完整的基因).