NCBI Primer-BLAST：A tool for finding specific primers
A tool for finding specific primers
NCBI Minute
https://www.youtube.com
Primer designing tool
https://www.ncbi.nlm.nih.gov
Primer-BLAST A tool for finding specific primers. Finding primers specific to your PCR template (using Primer3 and BLAST). Retrieve recent results ...
Primer
https://www.ncbi.nlm.nih.gov
Primer-BLAST to better identify the template and thus perform better primer specificity checking. A template is not required if both forward and reverse ...
Primer
https://www.ncbi.nlm.nih.gov
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target. It uses Primer3 to design PCR primers and then uses ...
Primer-BLAST
https://bmcbioinformatics.biom
Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers.
Using NCBI BLAST to locate primers
https://www.idtdna.com
Want to quickly find the location of primers within a gene or the expected size of the resultant PCR product? In this tip we show you how with NCBI's BLAST.
設計primer流程(捷登）
http://llai.cm.ntu.edu.tw
使⽤用時機：. 1. qPCR 時使⽤用（片段較短，<250 bp）. 2. ⽤用於⼀一般PCR 驗證. 3. ⽤用於質體建構時的target gene 製備(需恰好使⽤用整段完整的基因).