Cole1 copy number：resistance to RNA1 inhibition in vivo and in vitro
resistance to RNA1 inhibition in vivo and in vitro
由TFitzwater著作·1988·被引用53次—Thesemutantshave2-to4-foldincreasedcopynumberrelativetowild-typeplasmidsinexponentialgrowthat37degreesCbutundergo20-foldamplificationof ...。其他文章還包含有：「ColE1」、「Aplasmidsystemwithtunablecopynumber」、「Plasmids101」、「QuantitativemodelofColE1plasmidcopynumbercontrol」、「ColE1」、「Copynumbervariabilityofexpressionplasmidsdeterminedby...」、「Cole1e...
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ColE1
https://en.wikipedia.org
ColE1 has a copy number of 25-30 according to its original publication.
A plasmid system with tunable copy number
https://www.nature.com
We introduce two systems that allow for the continuous, finely-tuned control of plasmid copy number between 1 and 800 copies per cell.
Plasmids 101
https://blog.addgene.org
A plasmid's copy number has to do with the balance between positive and negative regulation and can be manipulated with mutations in the ...
Quantitative model of ColE1 plasmid copy number control
https://pubmed.ncbi.nlm.nih.go
We present a dynamic model for the in vivo replication control mechanism that accounts for the measured concentration values.
ColE1
https://www.sciencedirect.com
Interestingly, ColE1-derived cloning vectors like pUC18/19 replicate at increased copy numbers (200–300 copies per cell) due to a mutation in the rnaI gene that ...
Copy number variability of expression plasmids determined by ...
https://microbialcellfactories
The combined replication system pRO1600/ColE1 yielded a PCN of up to 40, which is in accordance with previous reports ranging between 15 and 50 ...
Cole 1 encodes a determinant essential for plasmid ...
https://www.sciencedirect.com
Article. Multimerization of high copy number plasmids causes instability: Cole 1 encodes a determinant essential for plasmid monomerization and stability.
Trans
https://www.nature.com
Here we show that deletion of a given non-essential region from ColE1-like plasmids results in a raised copy number.
How do I know if my plasmid is a high
https://www.qiagen.com
A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture.