Cole1 copy number：Plasmids 101
Plasmids 101
2014年2月6日—Aplasmid'scopynumberhastodowiththebalancebetweenpositiveandnegativeregulationandcanbemanipulatedwithmutationsinthe ...。其他文章還包含有：「ColE1」、「Aplasmidsystemwithtunablecopynumber」、「resistancetoRNA1inhibitioninvivoandinvitro」、「QuantitativemodelofColE1plasmidcopynumbercontrol」、「ColE1」、「Copynumbervariabilityofexpressionplasmidsdeterminedby...」、「Cole1encodesadet...
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ColE1
https://en.wikipedia.org
ColE1 has a copy number of 25-30 according to its original publication.
A plasmid system with tunable copy number
https://www.nature.com
We introduce two systems that allow for the continuous, finely-tuned control of plasmid copy number between 1 and 800 copies per cell.
resistance to RNA1 inhibition in vivo and in vitro
https://pubmed.ncbi.nlm.nih.go
These mutants have 2- to 4-fold increased copy number relative to wild-type plasmids in exponential growth at 37 degrees C but undergo 20-fold amplification of ...
Quantitative model of ColE1 plasmid copy number control
https://pubmed.ncbi.nlm.nih.go
We present a dynamic model for the in vivo replication control mechanism that accounts for the measured concentration values.
ColE1
https://www.sciencedirect.com
Interestingly, ColE1-derived cloning vectors like pUC18/19 replicate at increased copy numbers (200–300 copies per cell) due to a mutation in the rnaI gene that ...
Copy number variability of expression plasmids determined by ...
https://microbialcellfactories
The combined replication system pRO1600/ColE1 yielded a PCN of up to 40, which is in accordance with previous reports ranging between 15 and 50 ...
Cole 1 encodes a determinant essential for plasmid ...
https://www.sciencedirect.com
Article. Multimerization of high copy number plasmids causes instability: Cole 1 encodes a determinant essential for plasmid monomerization and stability.
Trans
https://www.nature.com
Here we show that deletion of a given non-essential region from ColE1-like plasmids results in a raised copy number.
How do I know if my plasmid is a high
https://www.qiagen.com
A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture.